Dataset Information

Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit⁺CD45^- cell population in newborns.

ABSTRACT:

Background

The aim of this study was to characterize the influence of cardiopulmonary bypass (CPB) on myocardial remodeling in newborns and children.

Methods

Biopsies from the right atrium were taken before and after CPB from 4 newborns (5-11 days old) and 7 children (8 months-16 years old). Immunostainings on 10 µm heart tissue frozen sections were performed to detect c-kit⁺ cells, leukocytes (CD45⁺ cells), Ki67⁺ cycling cells. The percentage of 8-hydroxy-guanosine (8-dOHG)⁺cardiomyocytes and non-cardiomyocytes [(8-dOHG)⁺-index] were determined to quantify oxidative stress.

Results

? c-kit⁺CD45^- cells (resident cardiac stem cells) were increased in newborns (2.2?±?1.9/mm²) and decreased in children -?1.5?±?0.7/mm², p?+-index was reduced by 43% in newborns and by 20% in children. CPB did not influence cardiac cell turnover; high cell proliferation was seen in newborns before and after CPB. Cardiopulmonary bypass significantly decreased the leucocyte infiltration in newborns to 40?±?8%, p?ConclusionsImmediate response and remodeling of the myocardium to CPB differs between newborns, older infants and children. Especially an increased number of c-kit expressing CD45 cells after CPB were seen in neonates in comparison to children. The clinical value of such observation needs to be further assessed in larger cohorts of patients.

SUBMITTER: Petersen J

PROVIDER: S-EPMC5921779 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Publications

Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit<sup>+</sup>CD45<sup>-</sup> cell population in newborns.

Petersen Johannes J Kazakov Andrey A Böhm Michael M Schäfers Hans-Joachim HJ Laufs Ulrich U Abdul-Khaliq Hashim H

Journal of translational medicine 20180427 1

<h4>Background</h4>The aim of this study was to characterize the influence of cardiopulmonary bypass (CPB) on myocardial remodeling in newborns and children.<h4>Methods</h4>Biopsies from the right atrium were taken before and after CPB from 4 newborns (5-11 days old) and 7 children (8 months-16 years old). Immunostainings on 10 µm heart tissue frozen sections were performed to detect c-kit<sup>+</sup> cells, leukocytes (CD45<sup>+</sup> cells), Ki67<sup>+</sup> cycling cells. The percentage of 8 ...[more]

PMID: 29703225

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Project description:ObjectivesChildren undergoing cardiopulmonary bypass develop clinically impactful capillary leak of unclear etiology. A widely held hypothesis that exposure of circulating cells to the cardiopulmonary bypass circuit induces the release of inflammatory mediators that act to disrupt intercellular junctions of capillary endothelial cells inducing paracellular capillary leak either directly or through new gene expression.DesignCohort study.SettingTertiary pediatric hospital.PatientsTwenty children undergoing surgery with cardiopulmonary bypass for congenital heart disease. Serum was collected before cardiopulmonary bypass, 2 hours after cardiopulmonary bypass, and 18 hours after cardiopulmonary bypass.InterventionsNone.Measurements and main resultsWe analyzed the effects of 10% patient sera on the "function, structure, and gene expression" of cultured human dermal and pulmonary microvascular endothelial cells. Changes in barrier "function" were measured using transendothelial electrical resistance. Associations between changes in transendothelial electrical resistance and subject characteristics were analyzed using linear mixed effects model with area under the resistance curve as outcome. Changes in junctional "structure" were assessed by analyzing the organization of the endothelial cell junctional proteins claudin-5 and VE-cadherin using immunofluorescence microscopy. Changes in inflammatory "gene expression" were measured using real-time quantitative reverse transcription-polymerase chain reaction. All serum samples induced a transient, 120-minute increase in transendothelial electrical resistance followed by persistent loss of barrier function. Unexpectedly, sera collected postcardiopulmonary bypass-induced significantly less loss of barrier function in both dermal and pulmonary capillary endothelial cell compared with precardiopulmonary bypass sera. Consistent with the transendothelial electrical resistance results, claudin-5 and vascular endothelial-cadherin junctional staining showed less disruption in cultures treated with postcardiopulmonary bypass sera. Expression of genes commonly associated with inflammation was largely unaffected by patient sera.ConclusionsContrary to the hypothesis, sera taken from children after cardiopulmonary bypass induces less capillary barrier disruption relative to sera taken from children before cardiopulmonary bypass, and none of the sera induced significant changes in expression of inflammatory genes.

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Dataset Information

Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit⁺CD45^- cell population in newborns.

Background

Methods

Results

Publications

Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit<sup>+</sup>CD45<sup>-</sup> cell population in newborns.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit+CD45- cell population in newborns.

Background

Methods

Results

Publications

Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit<sup>+</sup>CD45<sup>-</sup> cell population in newborns.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit⁺CD45^- cell population in newborns.