Ontology highlight
ABSTRACT:
SUBMITTER: Coradetti ST
PROVIDER: S-EPMC5922974 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Coradetti Samuel T ST Pinel Dominic D Geiselman Gina M GM Ito Masakazu M Mondo Stephen J SJ Reilly Morgann C MC Cheng Ya-Fang YF Bauer Stefan S Grigoriev Igor V IV Gladden John M JM Simmons Blake A BA Brem Rachel B RB Arkin Adam P AP Skerker Jeffrey M JM
eLife 20180309
The basidiomycete yeast <i>Rhodosporidium toruloides</i> (also known as <i>Rhodotorula toruloides</i>) accumulates high concentrations of lipids and carotenoids from diverse carbon sources. It has great potential as a model for the cellular biology of lipid droplets and for sustainable chemical production. We developed a method for high-throughput genetics (RB-TDNAseq), using sequence-barcoded <i>Agrobacterium tumefaciens</i> T-DNA insertions. We identified 1,337 putative essential genes with lo ...[more]