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Vesicle Tubulation with Self-Assembling DNA Nanosprings.

ABSTRACT: A major goal of nanotechnology and bioengineering is to build artificial nanomachines capable of generating specific membrane curvatures on demand. Inspired by natural membrane-deforming proteins, we designed DNA-origami curls that polymerize into nanosprings and show their efficacy in vesicle deformation. DNA-coated membrane tubules emerge from spherical vesicles when DNA-origami polymerization or high membrane-surface coverage occurs. Unlike many previous methods, the DNA self-assembly-mediated membrane tubulation eliminates the need for detergents or top-down manipulation. The DNA-origami design and deformation conditions have substantial influence on the tubulation efficiency and tube morphology, underscoring the intricate interplay between lipid bilayers and vesicle-deforming DNA structures.

SUBMITTER: Grome MW

PROVIDER: S-EPMC5924453 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Vesicle Tubulation with Self-Assembling DNA Nanosprings.

Grome Michael W MW Zhang Zhao Z Pincet Frédéric F Lin Chenxiang C

Angewandte Chemie (International ed. in English) 20180414 19

A major goal of nanotechnology and bioengineering is to build artificial nanomachines capable of generating specific membrane curvatures on demand. Inspired by natural membrane-deforming proteins, we designed DNA-origami curls that polymerize into nanosprings and show their efficacy in vesicle deformation. DNA-coated membrane tubules emerge from spherical vesicles when DNA-origami polymerization or high membrane-surface coverage occurs. Unlike many previous methods, the DNA self-assembly-mediate ...[more]

PMID: 29575478

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