Dataset Information

Sequence and Haplotypes Variation of the Ovine Uncoupling Protein-1 Gene (UCP1) and Their Association with Growth and Carcass Traits in New Zealand Romney Lambs.

ABSTRACT: Uncoupling protein-1 gene (UCP1) plays an important role in the regulation of thermogenesis, energy expenditure, and protection against oxidative stress. In this study, six separate UCP1 regions: region-1 and region-2 (two parts of the promoter), region-3 and region-4 (two parts of intron 1), region-5 (spanning part of intron 5 and part of exon 6), and region-6 (spanning part of exon 6 and part of the 3'-UTR) from a variety of sheep breeds, were analysed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) analyses. In total, 30 single nucleotide polymorphisms (SNPs) were detected. Of these, 14 were located in the promoter, eight were found in intron 1, six were found in intron 5, and one was found in the 3'-UTR. One substitution in exon 6 (c.910A/G) would putatively result in an amino acid change (p.Thr304Ala). Twenty-eight novel SNPs and nine new haplotypes spanning region-2 to region-5 were identified. Of these nine haplotypes, five were common (B?-A?, C?-A?, C?-C?, A?-A?, and A?-B?) and four were rare (C?-B?, A?-C?, B?-C?, and B?-B?) in the sheep investigated. Of the five common haplotypes found in 314 New Zealand Romney sheep for which growth and carcass trait data were available, the presence of A?-B? was associated with decreased hot carcass weight (HCW) and loin lean-meat yield (p = 0.006, p = 0.032, respectively), and the presence of C?-C? was associated with a decreased proportion of leg lean-meat yield (p = 0.047) in the carcasses. No associations were found with growth traits. These results confirm that ovine UCP1 is a variable gene and may have value as a genetic marker for sheep breeding.

SUBMITTER: An Q

PROVIDER: S-EPMC5924531 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Publications

Sequence and Haplotypes Variation of the Ovine Uncoupling Protein-1 Gene (UCP1) and Their Association with Growth and Carcass Traits in New Zealand Romney Lambs.

An Qingming Q Zhou Huitong H Hu Jiang J Luo Yuzhu Y Hickford Jon G H JGH

Genes 20180330 4

Uncoupling protein-1 gene (<i>UCP1</i>) plays an important role in the regulation of thermogenesis, energy expenditure, and protection against oxidative stress. In this study, six separate <i>UCP1</i> regions: region-1 and region-2 (two parts of the promoter), region-3 and region-4 (two parts of intron 1), region-5 (spanning part of intron 5 and part of exon 6), and region-6 (spanning part of exon 6 and part of the 3'-UTR) from a variety of sheep breeds, were analysed using polymerase chain reac ...[more]

PMID: 29601527

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Project description:Genetic parameters were estimated for growth, ultrasound, and carcass traits in a Canadian crossbred heavy lamb population. Traits analyzed included birth, weaning, post-weaning, and ultrasound scanning weights; pre- and post-weaning average daily gain; ultrasonically measured eye muscle and fat depths; hot carcass weight; fat depth at the GR site (110 mm from the midline on the 12th rib); carcass conformation scores; saleable meat yield; price grid value; and total carcass value. The impact of three alternative slaughter endpoints (slaughter age, carcass weight, and carcass fatness) on genetic parameter estimates was also evaluated. In general, carcass traits were found to be moderately heritable, with heritability estimates ranging from 0.17 ± 0.02 for hot carcass weight at a constant slaughter age to 0.34 ± 0.02 for average carcass conformation score at a constant carcass weight. Heritability estimates were similar when observations were adjusted to alternative slaughter endpoints, but for some traits, phenotypic variance and genetic correlation estimates differed. Genetic correlations between carcass traits and growth and ultrasound traits were typically favorable. Ultrasonically measured eye muscle depth and fat depth were found to be moderately to strongly positively correlated with hot carcass weight (0.33 ± 0.15 to 0.71 ± 0.19) and fat depth at the GR site (0.38 ± 0.14 to 0.74 ± 0.12), respectively, reaffirming the usefulness of selection on ultrasound traits to improve carcass yield and quality. Genetic correlations among carcass traits were generally favorable, with the exception of moderate unfavorable positive genetic correlations between fat depth at the GR site and primal cut carcass conformation scores (0.31 ± 0.05 to 0.60 ± 0.05). Overall, the results of this research suggest that there is potential to improve carcass yield and quality through genetic selection and provides the population-specific genetic parameter estimates needed for the genetic evaluation of carcass traits in the Canadian sheep population. Nevertheless, the optimal endpoint for carcass trait genetic evaluations will need to be further investigated, considering both the current findings and additional information on production practices in the industry.

Dataset Information

Sequence and Haplotypes Variation of the Ovine Uncoupling Protein-1 Gene (UCP1) and Their Association with Growth and Carcass Traits in New Zealand Romney Lambs.

Publications

Sequence and Haplotypes Variation of the Ovine Uncoupling Protein-1 Gene (UCP1) and Their Association with Growth and Carcass Traits in New Zealand Romney Lambs.

Similar Datasets

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