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Splicing and transcription touch base: co-transcriptional spliceosome assembly and function.

ABSTRACT: Several macromolecular machines collaborate to produce eukaryotic messenger RNA. RNA polymerase II (Pol II) translocates along genes that are up to millions of base pairs in length and generates a flexible RNA copy of the DNA template. This nascent RNA harbours introns that are removed by the spliceosome, which is a megadalton ribonucleoprotein complex that positions the distant ends of the intron into its catalytic centre. Emerging evidence that the catalytic spliceosome is physically close to Pol II in vivo implies that transcription and splicing occur on similar timescales and that the transcription and splicing machineries may be spatially constrained. In this Review, we discuss aspects of spliceosome assembly, transcription elongation and other co-transcriptional events that allow the temporal coordination of co-transcriptional splicing.

SUBMITTER: Herzel L

PROVIDER: S-EPMC5928008 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Splicing and transcription touch base: co-transcriptional spliceosome assembly and function.

Herzel Lydia L Ottoz Diana S M DSM Alpert Tara T Neugebauer Karla M KM

Nature reviews. Molecular cell biology 20170809 10

Several macromolecular machines collaborate to produce eukaryotic messenger RNA. RNA polymerase II (Pol II) translocates along genes that are up to millions of base pairs in length and generates a flexible RNA copy of the DNA template. This nascent RNA harbours introns that are removed by the spliceosome, which is a megadalton ribonucleoprotein complex that positions the distant ends of the intron into its catalytic centre. Emerging evidence that the catalytic spliceosome is physically close to ...[more]

PMID: 28792005

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2019-06-25 | GSE120051 | GEO

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