Dataset Information

Genome-wide Identification and characterization of circular RNAs in the rice blast fungus Magnaporthe oryzae.

ABSTRACT: Numerous circRNAs have been identified in different organisms, but little attention has been addressed on fungal circRNAs. Here, we identified a total of 8,848 circRNAs from the model plant pathogenic fungus M. oryzae. 5,840 circRNAs were identified from mycelium, 2,721 circRNAs from conidium, while only 287 circRNAs from both tissues. This indicated that most of the M. oryzae circRNAs were specifically expressed in mycelium or in conidium. Parental genes of circRNAs in mycelium were enriched in basic metabolisms required for normal growth, while in conidium, they were enriched in biogenesis of storages potentially used for infection. M. oryzae circRNAs could also bind to miRNAs, suggesting they may also function as sponges in fungi. This study suggested M. oryzae circRNAs could play important roles in regulation of growth and development.

SUBMITTER: Yuan J

PROVIDER: S-EPMC5928111 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Publications

Genome-wide Identification and characterization of circular RNAs in the rice blast fungus Magnaporthe oryzae.

Yuan Jialan J Wang Zhao Z Xing Junjie J Yang Qingyong Q Chen Xiao-Lin XL

Scientific reports 20180430 1

Numerous circRNAs have been identified in different organisms, but little attention has been addressed on fungal circRNAs. Here, we identified a total of 8,848 circRNAs from the model plant pathogenic fungus M. oryzae. 5,840 circRNAs were identified from mycelium, 2,721 circRNAs from conidium, while only 287 circRNAs from both tissues. This indicated that most of the M. oryzae circRNAs were specifically expressed in mycelium or in conidium. Parental genes of circRNAs in mycelium were enriched in ...[more]

PMID: 29713025

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Project description:BACKGROUND: Magnaporthe oryzae, the causal agent of blast disease of rice, is the most destructive disease of rice worldwide. The genome of this fungal pathogen has been sequenced and an automated annotation has recently been updated to Version 6 http://www.broad.mit.edu/annotation/genome/magnaporthe_grisea/MultiDownloads.html. However, a comprehensive manual curation remains to be performed. Gene Ontology (GO) annotation is a valuable means of assigning functional information using standardized vocabulary. We report an overview of the GO annotation for Version 5 of M. oryzae genome assembly. METHODS: A similarity-based (i.e., computational) GO annotation with manual review was conducted, which was then integrated with a literature-based GO annotation with computational assistance. For similarity-based GO annotation a stringent reciprocal best hits method was used to identify similarity between predicted proteins of M. oryzae and GO proteins from multiple organisms with published associations to GO terms. Significant alignment pairs were manually reviewed. Functional assignments were further cross-validated with manually reviewed data, conserved domains, or data determined by wet lab experiments. Additionally, biological appropriateness of the functional assignments was manually checked. RESULTS: In total, 6,286 proteins received GO term assignment via the homology-based annotation, including 2,870 hypothetical proteins. Literature-based experimental evidence, such as microarray, MPSS, T-DNA insertion mutation, or gene knockout mutation, resulted in 2,810 proteins being annotated with GO terms. Of these, 1,673 proteins were annotated with new terms developed for Plant-Associated Microbe Gene Ontology (PAMGO). In addition, 67 experiment-determined secreted proteins were annotated with PAMGO terms. Integration of the two data sets resulted in 7,412 proteins (57%) being annotated with 1,957 distinct and specific GO terms. Unannotated proteins were assigned to the 3 root terms. The Version 5 GO annotation is publically queryable via the GO site http://amigo.geneontology.org/cgi-bin/amigo/go.cgi. Additionally, the genome of M. oryzae is constantly being refined and updated as new information is incorporated. For the latest GO annotation of Version 6 genome, please visit our website http://scotland.fgl.ncsu.edu/smeng/GoAnnotationMagnaporthegrisea.html. The preliminary GO annotation of Version 6 genome is placed at a local MySql database that is publically queryable via a user-friendly interface Adhoc Query System. CONCLUSION: Our analysis provides comprehensive and robust GO annotations of the M. oryzae genome assemblies that will be solid foundations for further functional interrogation of M. oryzae.

Dataset Information

Genome-wide Identification and characterization of circular RNAs in the rice blast fungus Magnaporthe oryzae.

Publications

Genome-wide Identification and characterization of circular RNAs in the rice blast fungus Magnaporthe oryzae.

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