Unknown

Dataset Information

0

Deep Top-Down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry: Identification of 5700 Proteoforms from the Escherichia coli Proteome.


ABSTRACT: Capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) has been recognized as a useful tool for top-down proteomics. However, its performance for deep top-down proteomics is still dramatically lower than widely used reversed-phase liquid chromatography (RPLC)-MS/MS. We present an orthogonal multidimensional separation platform that couples size exclusion chromatography (SEC) and RPLC based protein prefractionation to CZE-MS/MS for deep top-down proteomics of Escherichia coli. The platform generated high peak capacity (∼4000) for separation of intact proteins, leading to the identification of 5700 proteoforms from the Escherichia coli proteome. The data represents a 10-fold improvement in the number of proteoform identifications compared with previous CZE-MS/MS studies and represents the largest bacterial top-down proteomics data set reported to date. The performance of the CZE-MS/MS based platform is comparable to the state-of-the-art RPLC-MS/MS based systems in terms of the number of proteoform identifications and the instrument time.

SUBMITTER: McCool EN 

PROVIDER: S-EPMC5930140 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Deep Top-Down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry: Identification of 5700 Proteoforms from the Escherichia coli Proteome.

McCool Elijah N EN   Lubeckyj Rachele A RA   Shen Xiaojing X   Chen Daoyang D   Kou Qiang Q   Liu Xiaowen X   Sun Liangliang L  

Analytical chemistry 20180409 9


Capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) has been recognized as a useful tool for top-down proteomics. However, its performance for deep top-down proteomics is still dramatically lower than widely used reversed-phase liquid chromatography (RPLC)-MS/MS. We present an orthogonal multidimensional separation platform that couples size exclusion chromatography (SEC) and RPLC based protein prefractionation to CZE-MS/MS for deep top-down proteomics of Escherichia coli. The  ...[more]

Similar Datasets

| S-EPMC5726253 | biostudies-literature
| S-EPMC6557293 | biostudies-literature
| S-EPMC10540247 | biostudies-literature
| S-EPMC10922523 | biostudies-literature
| S-EPMC6675661 | biostudies-literature
| S-EPMC6788745 | biostudies-literature
| S-EPMC11002928 | biostudies-literature
| S-EPMC6527361 | biostudies-literature
| S-EPMC8564867 | biostudies-literature
| S-EPMC10540249 | biostudies-literature