Dataset Information

An endogenous microRNA (miRNA1166.1) can regulate photobio-H₂ production in eukaryotic green alga Chlamydomonas reinhardtii.

ABSTRACT:

Background

Hydrogen photoproduction from green microalgae is regarded as a promising alternative solution for energy problems. However, the simultaneous oxygen evolution from microalgae can prevent continuous hydrogen production due to the hypersensitivity of hydrogenases to oxygen. Sulfur deprivation can extend the duration of algal hydrogen production, but it is uneconomical to alternately culture algal cells in sulfur-sufficient and sulfur-deprived media.

Results

In this study, we developed a novel way to simulate sulfur-deprivation treatment while constantly maintaining microalgal cells in sulfur-sufficient culture medium by overexpressing an endogenous microRNA (miR1166.1). Based on our previous RNA-seq analysis in the model green alga Chlamydomonas reinhardtii, three endogenous miRNAs responsive to sulfur deprivation (cre-miR1166.1, cre-miR1150.3, and cre-miR1158) were selected. Heat-inducible expression vectors containing the selected miRNAs were constructed and transformed into C. reinhardtii. Comparison of H₂ production following heat induction in the three transgenic strains and untransformed control group identified miR1166.1 as the best candidate for H₂ production regulation. Moreover, enhanced photobio-H₂ production was observed with repeated induction of miR1166.1 expression.

Conclusions

This study is the first to identify a physiological function of endogenous miR1166.1 and to show that a natural miRNA can regulate hydrogen photoproduction in the unicellular model organism C. reinhardtii.

SUBMITTER: Wang Y

PROVIDER: S-EPMC5930490 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

An endogenous microRNA (miRNA1166.1) can regulate photobio-H<sub>2</sub> production in eukaryotic green alga <i>Chlamydomonas reinhardtii</i>.

Wang Yuting Y Zhuang Xiaoshan X Chen Meirong M Zeng Zhiyong Z Cai Xiaoqi X Li Hui H Hu Zhangli Z

Biotechnology for biofuels 20180502

<h4>Background</h4>Hydrogen photoproduction from green microalgae is regarded as a promising alternative solution for energy problems. However, the simultaneous oxygen evolution from microalgae can prevent continuous hydrogen production due to the hypersensitivity of hydrogenases to oxygen. Sulfur deprivation can extend the duration of algal hydrogen production, but it is uneconomical to alternately culture algal cells in sulfur-sufficient and sulfur-deprived media.<h4>Results</h4>In this study, ...[more]

PMID: 29743954

Similar Datasets

Project description:Algal photo-bio hydrogen production, a promising method for producing clean and renewable fuel in the form of hydrogen gas, has been studied extensively over the last few decades. In this study, microarray analyses were used to obtain a global expression profile of mRNA abundance in the green alga Chlamydomonas reinhardtii at five different time points before the onset and during the course of sulphur depleted hydrogen production. The present work confirms previous findings on the impacts of sulphur deprivation but also provides new insights into photosynthesis, sulphur assimilation and carbon metabolism under sulphur starvation towards hydrogen production. For instance, while a general trend towards repression of transcripts encoding photosynthetic genes was observed, the abundance of Lhcbm9 (encoding a major light harvesting polypeptide) and LhcSR1 (encoding a chlorophyll binding protein) was strongly elevated throughout the experiment, suggesting remodeling of the photosystem II light harvesting complex as well as an important function of Lhcbm9 under sulphur starvation. This study presents the first global transcriptional analysis of C. reinhardtii during hydrogen production using five major time points at Peak Oxygen, Mid Oxygen, Zero Oxygen, Mid Hydrogen and Peak Hydrogen. Keywords: Time course, sulfur deprivation, hydrogen production. Time course microarray analyses were used to analyze the global gene expression in sulfur depleted hydrogen producing C. reinhardtii. When depleted of sulfur, a sealed an illuminated C. reinhardtii culture slowly becomes anaerobic and produces hydrogen gas. Based on the changes in the dissolved O2 level and H2 production rate, the whole course of H2 production from the starting of S deprivation to the end of H2 production can be divided into five phases including an Aerobic Phase (I) in which the dissolved O2 level goes up (I), an O2 Consumption Phase (II), an Anaerobic Phase (III), a H2 Production Phase (IV) and a Termination phase (V). Samples were taken from three different bioreactors (biological replicates) at the following time points after the start of S depletion: 6 h, 16 h, 21 h, 37 h and 52 h corresponding to Peak O2, Mid O2, Zero O2, Mid H2 and Peak H2.

Dataset Information

An endogenous microRNA (miRNA1166.1) can regulate photobio-H₂ production in eukaryotic green alga Chlamydomonas reinhardtii.

Background

Results

Conclusions

Publications

An endogenous microRNA (miRNA1166.1) can regulate photobio-H<sub>2</sub> production in eukaryotic green alga <i>Chlamydomonas reinhardtii</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

An endogenous microRNA (miRNA1166.1) can regulate photobio-H2 production in eukaryotic green alga Chlamydomonas reinhardtii.

Background

Results

Conclusions

Publications

An endogenous microRNA (miRNA1166.1) can regulate photobio-H<sub>2</sub> production in eukaryotic green alga <i>Chlamydomonas reinhardtii</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

An endogenous microRNA (miRNA1166.1) can regulate photobio-H₂ production in eukaryotic green alga Chlamydomonas reinhardtii.