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Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods.


ABSTRACT: In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage showed that it had a 33,697-bp double-stranded DNA genome with 41.2% G+C content. Bioinformatics analysis of the genome revealed 35 open reading frames. A BLASTN search showed that WCP30 phage had low sequence similarity compared to other phages infecting lactic acid bacteria. This is the first report of the morphological features and complete genome sequence of WCP30 phage, which may be useful for controlling the fermentation of dairy foods.

SUBMITTER: Lee YD 

PROVIDER: S-EPMC5932951 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Genomic analysis of WCP30 Phage of <i>Weissella cibaria</i> for Dairy Fermented Foods.

Lee Young-Duck YD   Park Jong-Hyun JH  

Korean journal for food science of animal resources 20171231 6


In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of <i>Weissella cibaria</i>, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family <i>Siphoviridae</i>. Genomic analysis of WCP30 phage showed that it had a 33,697-bp double-stranded DNA genome with 41.2% G+C content. Bioinformatics analysis of the genome revealed 35 open reading frames. A BLASTN search showed that WCP3  ...[more]

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