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RSM based optimization of nutritional conditions for cellulase mediated Saccharification by Bacillus cereus.

ABSTRACT: Background:Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of Bacillus cereus was explored while attacking poplar twigs. The bacterium was isolated from the gut of freshwater fish, Labeo rohita and identified by 16S rRNA gene sequencing technology. Various nutritional conditions were screened and optimized through response surface methodology. Initially, Plackett-Burman design was used for screening purpose and optimization was conducted through Box-Bhenken design. Results:The maximum cellulase production occurred at 0.5% yeast extract, 0.09% MgSO4, 0.04% peptone, 2% poplar waste biomass, initial medium pH of 9.0, and inoculum size of 2% v/v at 37 °C with agitation speed of 120 rpm for 24 h of submerged fermentation. The proposed model for optimization of cellulase production was found highly significant. The indigenously produced cellulase enzyme was employed for saccharification purpose at 50 °C for various time periods. Maximum total sugars of 31.42 mg/ml were released after 6 h of incubation at 50 °C.The efficiency of this enzyme was compared with commercial cellulase enzyme revealing significant findings. Conclusion:These results suggested potential utilization of this strain in biofuel industry.

SUBMITTER: Tabssum F

PROVIDER: S-EPMC5934882 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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RSM based optimization of nutritional conditions for cellulase mediated Saccharification by <i>Bacillus cereus</i>.

Tabssum Fouzia F Irfan Muhammad M Shakir Hafiz Abdullah HA Qazi Javed Iqbal JI

Journal of biological engineering 20180503

<h4>Background</h4>Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of <i>Bacillus cereus</i> was explored while attacking poplar twigs. The bacterium was isolated from the gut of freshwater fish, <i>Labeo rohita</i> and identified by 16S rRNA gene sequencing technology. Various nutritional conditions were screened and optimiz ...[more]

PMID: 29755582

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Project description:BackgroundThe extracellular xylanase secreted by microorganisms is a hydrolytic enzyme, which arbitrarily cleaves the β-1, 4 backbone of the polysaccharide xylan; an enzyme used in the food processing, bio-pulping and bio-bleaching. The commercial production of the xylanase is limited because of a higher cost involvement, which can be overcome by the cost-effective production of the xylanase through immobilization of the microbial cell by the non-toxic substances.ObjectivesIn this work, the optimization of the extra-cellular cellulase free xylanase production by the immobilized cell of the Bacillus pumilus IMAU80221 strain using Ca-alginate beads along with standardization of the various parameters for a higher xylanase production were studied.Materials and methodsFollowing to sterilization, the Na-alginate solution was mixed with the bacterial suspension of the Bacillus pumilus IMAU80221 and was added drop by drop into the 1 M calcium chloride solution for 1 h for obtaining a uniform sized polymeric bead of the Ca-alginate. For xylanase production, the Ca-alginate beads were then transferred into 100 mL Erlenmeyer flasks with 20 mL of the culture medium containing (w/v) 0.02% NaCl, 0.02% MgSO4, 0.04% (KH4)2PO4, 0.1% peptone, and 0.5% xylan and incubated at 34 °C in an incubator shaker (150 rpm) for 24 h. The resultant supernatant (crude enzyme) was used for enzyme assay.ResultsThe maximum xylanase production by the free cell (1.9 U.mL-1.min-1) was recorded at 48 h which was 40.5% lower than the xylanase production by the immobilized cell (2.67 U.mL-1.min-1) at the same time. The beads containing the immobilized cells could be reused up to eight fermentation cycles for xylanase production and retained 83.5% of the productivity at the fourth cycle. The entrapped cells were stable after six months of storage at 4 °C and retained 68% of the xylanase productivity.ConclusionCellulase free xylanase production from the immobilized Bacillus pumilus IMAU80221 was optimized. The xylanase production by the immobilized cells of Bacillus pumilus was higher by 40.5 and 132.6 % over the free cells respectively after 48 and 72 h of the incubation.

Dataset Information

RSM based optimization of nutritional conditions for cellulase mediated Saccharification by Bacillus cereus.

Publications

RSM based optimization of nutritional conditions for cellulase mediated Saccharification by <i>Bacillus cereus</i>.

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