Phase Transitions in the Assembly and Function of Human miRISC.
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ABSTRACT:
SUBMITTER: Sheu-Gruttadauria J
PROVIDER: S-EPMC5935535 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Publications
Phase Transitions in the Assembly and Function of Human miRISC.
Sheu-Gruttadauria Jessica J MacRae Ian J IJ
Cell 20180322 4
miRISC is a multi-protein assembly that uses microRNAs (miRNAs) to identify mRNAs targeted for repression. Dozens of miRISC-associated proteins have been identified, and interactions between many factors have been examined in detail. However, the physical nature of the complex remains unknown. Here, we show that two core protein components of human miRISC, Argonaute2 (Ago2) and TNRC6B, condense into phase-separated droplets in vitro and in live cells. Phase separation is promoted by multivalent ...[more]
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