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Generation of App knock-in mice reveals deletion mutations protective against Alzheimer's disease-like pathology.


ABSTRACT: Although, a number of pathogenic mutations have been found for Alzheimer's disease (AD), only one protective mutation has been identified so far in humans. Here we identify possible protective deletion mutations in the 3'-UTR of the amyloid precursor protein (App) gene in mice. We use an App knock-in mouse model carrying a humanized A? sequence and three AD mutations in the endogenous App gene. Genome editing of the model zygotes using multiple combinations of CRISPR/Cas9 tools produces genetically mosaic animals with various App 3'-UTR deletions. Depending on the editing efficiency, the 3'-UTR disruption mitigates the A? pathology development through transcriptional and translational regulation of APP expression. Notably, an App knock-in mouse with a 34-bp deletion in a 52-bp regulatory element adjacent to the stop codon shows a substantial reduction in A? pathology. Further functional characterization of the identified element should provide deeper understanding of the pathogenic mechanisms of AD.

SUBMITTER: Nagata K 

PROVIDER: S-EPMC5935712 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Generation of App knock-in mice reveals deletion mutations protective against Alzheimer's disease-like pathology.

Nagata Kenichi K   Takahashi Mika M   Matsuba Yukio Y   Okuyama-Uchimura Fumi F   Sato Kaori K   Hashimoto Shoko S   Saito Takashi T   Saido Takaomi C TC  

Nature communications 20180504 1


Although, a number of pathogenic mutations have been found for Alzheimer's disease (AD), only one protective mutation has been identified so far in humans. Here we identify possible protective deletion mutations in the 3'-UTR of the amyloid precursor protein (App) gene in mice. We use an App knock-in mouse model carrying a humanized Aβ sequence and three AD mutations in the endogenous App gene. Genome editing of the model zygotes using multiple combinations of CRISPR/Cas9 tools produces genetica  ...[more]

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