Ontology highlight
ABSTRACT:
SUBMITTER: Sriramoju MK
PROVIDER: S-EPMC5935755 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Scientific reports 20180504 1
More than one thousand knotted protein structures have been identified so far, but the functional roles of these knots remain elusive. It has been postulated that backbone entanglement may provide additional mechanostability. Here, we employed a bacterial proteasome, ClpXP, to mechanically unfold 5<sub>2</sub>-knotted human ubiquitin C-terminal hydrolase (UCH) paralogs from their C-termini, followed by processive translocation into the proteolytic chamber for degradation. Our results revealed un ...[more]