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Flutter to tumble transition of buoyant spheres triggered by rotational inertia changes.

ABSTRACT: Heavy particles sink straight in water, while buoyant bubbles and spheres may zigzag or spiral as they rise. The precise conditions that trigger such path-instabilities are still not completely understood. For a buoyant rising sphere, two parameters are believed to govern the development of unsteady dynamics: the particle's density relative to the fluid, and its Galileo number. Consequently, with these parameters specified, the opportunities for variation in particle dynamics appear limited. In contrast to this picture, here we demonstrate that vigorous path-oscillations can be triggered by modulating a spherical particle's moment of inertia (MoI). For a buoyant sphere rising in a turbulent flow, MoI reduction triggers a tumble-flutter transition, while in quiescent liquid, it induces a modification of the sphere wake resulting in large-amplitude path-oscillations. The present finding opens the door for control of particle path- and wake-instabilities, with potential for enhanced mixing and heat transfer in particle-laden and dispersed multiphase environments.

SUBMITTER: Mathai V

PROVIDER: S-EPMC5935758 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Flutter to tumble transition of buoyant spheres triggered by rotational inertia changes.

Mathai Varghese V Zhu Xiaojue X Sun Chao C Lohse Detlef D

Nature communications 20180504 1

Heavy particles sink straight in water, while buoyant bubbles and spheres may zigzag or spiral as they rise. The precise conditions that trigger such path-instabilities are still not completely understood. For a buoyant rising sphere, two parameters are believed to govern the development of unsteady dynamics: the particle's density relative to the fluid, and its Galileo number. Consequently, with these parameters specified, the opportunities for variation in particle dynamics appear limited. In ...[more]

PMID: 29728557

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Project description:Abortive cycling is a universal feature of transcription initiation catalyzed by DNA-dependent RNA polymerases (RNAP). In bacteriophage T7 RNAP, mutation of proline 266 to leucine (P266L) in the C-linker region connecting the N-terminal promoter binding domain with the C-terminal catalytic domain drastically reduces short abortive products (4-7 nt) while marginally increasing long abortives (9-11 nt). Here we have investigated the transcription initiation pathway of P266L with the goal of understanding the mechanistic basis for short and long abortive synthesis. We show that the P266L mutation does not alter the affinity for the promoter, mildly affects promoter opening, and increases the +1/+2 GTP K(d) by 2-fold. However, unlike wild-type T7 RNAP that undergoes stepwise rotation of the promoter binding domain and DNA scrunching during initial transcription, the P266L mutant does not undergo coupled rotational/scrunching movements until 7 nt RNA synthesis. The lack of rotation/scrunching correlates with greater stabilities of the initiation complexes of the P266L and decreased short abortive products. The results indicate that the increased flexibility in the C-linker due to P266L mutation enables T7 RNAP to absorb the stress from the growing RNA:DNA hybrid thereby decreasing short abortive products. Increased C-linker flexibility, however, has an adverse effect of delaying the transition into elongation by 1-2 nt, which gives rise to long abortive products. However, a mutation in the upstream promoter region greatly decreases long abortive products in P266L reactions, rendering the combination of P266L and A-15C promoter a desirable pair for efficient in vitro transcription for RNA production. We conclude that the conformational rigidity in the C-linker region conferred by the proline at position 266 is responsible for the undesirable short abortive products, but the rigidity is critical for efficient promoter clearance and transition into elongation.

Dataset Information

Flutter to tumble transition of buoyant spheres triggered by rotational inertia changes.

Publications

Flutter to tumble transition of buoyant spheres triggered by rotational inertia changes.

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