Unknown

Dataset Information

0

Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein.


ABSTRACT: Dengue virus (DENV) is the most prevalent and burdensome arbovirus transmitted by Aedes mosquitoes, against which there is only a limited licensed vaccine and no approved drug treatment. A Chromobacterium species, C. sp. Panama, isolated from the midgut of A. aegypti is able to inhibit DENV replication within the mosquito and in vitro. Here we show that C. sp. Panama mediates its anti-DENV activity through secreted factors that are proteinous in nature. The inhibitory effect occurs prior to virus attachment to cells, and is attributed to a factor that destabilizes the virion by promoting the degradation of the viral envelope protein. Bioassay-guided fractionation, coupled with mass spectrometry, allowed for the identification of a C. sp. Panama-secreted neutral protease and an aminopeptidase that are co-expressed and appear to act synergistically to degrade the viral envelope (E) protein and thus prevent viral attachment and subsequent infection of cells. This is the first study characterizing the anti-DENV activity of a common soil and mosquito-associated bacterium, thereby contributing towards understanding how such bacteria may limit disease transmission, and providing new tools for dengue prevention and therapeutics.

SUBMITTER: Saraiva RG 

PROVIDER: S-EPMC5937796 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein.

Saraiva Raúl G RG   Fang Jingru J   Kang Seokyoung S   Angleró-Rodríguez Yesseinia I YI   Dong Yuemei Y   Dimopoulos George G  

PLoS neglected tropical diseases 20180425 4


Dengue virus (DENV) is the most prevalent and burdensome arbovirus transmitted by Aedes mosquitoes, against which there is only a limited licensed vaccine and no approved drug treatment. A Chromobacterium species, C. sp. Panama, isolated from the midgut of A. aegypti is able to inhibit DENV replication within the mosquito and in vitro. Here we show that C. sp. Panama mediates its anti-DENV activity through secreted factors that are proteinous in nature. The inhibitory effect occurs prior to viru  ...[more]

Similar Datasets

| S-EPMC7237781 | biostudies-literature
| S-EPMC5887189 | biostudies-literature
| S-EPMC5974309 | biostudies-literature
2018-03-31 | GSE109727 | GEO
| S-EPMC8067805 | biostudies-literature
| S-EPMC8070382 | biostudies-literature
2011-10-31 | E-GEOD-33274 | biostudies-arrayexpress
| S-EPMC8708800 | biostudies-literature
2011-10-31 | GSE33274 | GEO
| S-EPMC3145677 | biostudies-literature