Ontology highlight
ABSTRACT:
SUBMITTER: Zhao R
PROVIDER: S-EPMC5940202 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Zhao Ruili R Zhao Rui R Tu Yishuai Y Zhang Xiaoming X Deng Liping L Chen Xiangdong X
PloS one 20180508 5
A novel α-galactosidase of glycoside hydrolase family 36 was cloned from Bacillus coagulans, overexpressed in Escherichia coli, and characterized. The purified enzyme Aga-BC7050 was 85 kDa according to SDS-PAGE and 168 kDa according to gel filtration, indicating that its native structure is a dimer. With p-nitrophenyl-α-d- galactopyranoside (pNPGal) as the substrate, optimal temperature and pH were 55 °C and 6.0, respectively. At 60 °C for 30 min, it retained > 50% of its activity. It was stable ...[more]