Ontology highlight
ABSTRACT:
SUBMITTER: Rayaprolu V
PROVIDER: S-EPMC5940254 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Rayaprolu Vamseedhar V Royal Perrine P Stengel Karen K Sandoz Guillaume G Kohout Susy C SC
The Journal of general physiology 20180425 5
Multimerization is a key characteristic of most voltage-sensing proteins. The main exception was thought to be the <i>Ciona intestinalis</i> voltage-sensing phosphatase (Ci-VSP). In this study, we show that multimerization is also critical for Ci-VSP function. Using coimmunoprecipitation and single-molecule pull-down, we find that Ci-VSP stoichiometry is flexible. It exists as both monomers and dimers, with dimers favored at higher concentrations. We show strong dimerization via the voltage-sens ...[more]