Dataset Information

Complete Genome Sequencing of a Classical Swine Fever Virus Strain Endemic in Vietnam.

ABSTRACT: A Vietnamese strain of classical swine fever virus, VN91, was isolated in Hung Yen in 1991. While VN91 has been used as a challenge strain in efficacy tests of vaccines, its genetic background has never been described. Here, we report the genome sequence of the strain circulating in Vietnam.

SUBMITTER: Tran HT

PROVIDER: S-EPMC5940935 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Complete Genome Sequencing of a Classical Swine Fever Virus Strain Endemic in Vietnam.

Tran Ha Thanh HT Dang Hoang Vu HV Nguyen Dung Tien DT Miyazawa Kohtaro K Kokuho Takehiro T

Genome announcements 20180503 18

A Vietnamese strain of classical swine fever virus, VN91, was isolated in Hung Yen in 1991. While VN91 has been used as a challenge strain in efficacy tests of vaccines, its genetic background has never been described. Here, we report the genome sequence of the strain circulating in Vietnam. ...[more]

PMID: 29724831

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Project description:Recently moderate-virulence classical swine fever virus (CSFV) strains have been proven capable of generating postnatal persistent infection (PI), defined by the maintenance of viremia and the inability to generate CSFV-specific immune responses in animals. These animals also showed a type I interferon blockade in the absence of clinical signs. In this study, we assessed the infection generated in 7-week-old CSFV PI wild boars after infection with the African swine fever virus (ASFV). The wild boars were divided in two groups and were infected with ASFV. Group A comprised boars who were CSFV PI in a subclinical form and Group B comprised pestivirus-free wild boars. Some relevant parameters related to CSFV replication and the immune response of CSFV PI animals were studied. Additionally, serum soluble factors such as IFN-α, TNF-α, IL-6, IL-10, IFN-γ and sCD163 were analysed before and after ASFV infection to assess their role in disease progression.After ASFV infection, only the CSFV PI wild boars showed progressive acute haemorrhagic disease; however, the survival rates following ASFV infection was similar in both experimental groups. Notwithstanding, the CSFV RNA load of CSFV PI animals remained unaltered over the study; likewise, the ASFV DNA load detected after infection was similar between groups. Interestingly, systemic type I FN-α and IL-10 levels in sera were almost undetectable in CSFV PI animals, yet detectable in Group B, while detectable levels of IFN-γ were found in both groups. Finally, the flow cytometry analysis showed an increase in myelomonocytic cells (CD172a+) and a decrease in CD4+ T cells in the PBMCs from CSFV PI animals after ASFV infection.Our results showed that the immune response plays a role in the progression of disease in CSFV subclinically infected wild boars after ASFV infection, and the immune response comprised the systemic type I interferon blockade. ASFV does not produce any interference with CSFV replication, or vice versa. ASFV infection could be a trigger factor for the disease progression in CSFV PI animals, as their survival after ASFV was similar to that of the pestivirus-free ASFV-infected group. This fact suggests a high resistance in CSFV PI animals even against a virus like ASFV; this may mean that there are relevant implications for CSF control in endemic countries. The diagnosis of ASFV and CSFV co-infection in endemic countries cannot be ruled out and need to be studied in greater depth.

Dataset Information

Complete Genome Sequencing of a Classical Swine Fever Virus Strain Endemic in Vietnam.

Publications

Complete Genome Sequencing of a Classical Swine Fever Virus Strain Endemic in Vietnam.

Similar Datasets

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