Ontology highlight
ABSTRACT:
SUBMITTER: Bontemps-Gallo S
PROVIDER: S-EPMC5943160 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Bontemps-Gallo Sébastien S Lawrence Kevin A KA Richards Crystal L CL Gherardini Frank C FC
Molecular microbiology 20180311 4
Polyamines are an essential class of metabolites found throughout all kingdoms in life. Borrelia burgdorferi harbors no enzymes to synthesize or degrade polyamines yet does contain a polyamine uptake system, potABCD. In this report, we describe the initial characterization of this putative transport system. After several unsuccessful attempts to inactivate potABCD, we placed the operon under the control of an inducible LacI promoter expression system. Analyses of this construct confirmed that po ...[more]