LncRNA-NKILA/NF-?B feedback loop modulates laryngeal cancer cell proliferation, invasion, and radioresistance.

ABSTRACT: Laryngeal cancer is one of the most common head and neck malignant tumors and is commonly resistant to X-ray-based radiotherapy. NF-?B interacting lncRNA (NKILA) has been reported to serve as a tumor suppressor in several cancers through combining with NF-?B: I?B complex thereby inhibiting NF-?B activation. Herein, we demonstrated a low NKILA expression in laryngeal cancer and its correlation with shorter overall survival in patients with laryngeal cancer. NKILA serves as a tumor suppressor in laryngeal cancer by suppressing laryngeal cancer cell viability and migration, whereas promoting cell apoptosis; NKILA knockdown reverses the cytotoxicity of X-ray radiation on laryngeal cancer cells through combining with NF-?B: I?B complex to inhibit I?B phosphorylation, inhibit p65 nuclear translocation, and finally inhibit NF-?B activation. NF-?B binds to the promoter region of NKILA to activate its transcriptional activity, upregulated NKILA then inhibits I?B phosphorylation and NF-?B activation, thus forming a negative feedback loop to sensitize laryngeal cancer cell to X-ray radiation. In conclusion, NKILA can serve as a promising agent of enhancing the cytotoxicity of X-ray radiation on laryngeal cancer and addressing the radioresistance of laryngeal cancer.

SUBMITTER: Yang T 

PROVIDER: S-EPMC5943486 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature