Dataset Information

Development and characterization of simple sequence repeat markers for the invasive tetraploid waterweed Elodea nuttallii (Hydrocharitaceae).

ABSTRACT:

Premise of the study

To enhance the understanding of the recent invasion process of the clonal waterweed Elodea nuttallii (Hydrocharitaceae), analyses of population structure and genotypic diversity need to be undertaken, for which genetic markers are needed.

Methods and results

High-throughput sequencing of DNA enriched for microsatellites was used to develop 24 loci that were characterized in E. nuttallii, 21 of which were polymorphic, with the number of alleles ranging from two to 10. In two populations, expected heterozygosity ranged among loci between zero and 0.796. In the congener E. canadensis, all markers yielded PCR products, 19 of which were polymorphic, with two to nine alleles and expected heterozygosity ranging from zero to 0.690 in two populations.

Conclusions

The markers described should be useful for future studies of population structure and clonal diversity of E. nuttallii as well as E. canadensis in their native and invasive range.

SUBMITTER: Weickardt I

PROVIDER: S-EPMC5947605 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Publications

Development and characterization of simple sequence repeat markers for the invasive tetraploid waterweed <i>Elodea nuttallii</i> (Hydrocharitaceae).

Weickardt Isabell I Zehnsdorf Andreas A Durka Walter W

Applications in plant sciences 20180401 4

<h4>Premise of the study</h4>To enhance the understanding of the recent invasion process of the clonal waterweed <i>Elodea nuttallii</i> (Hydrocharitaceae), analyses of population structure and genotypic diversity need to be undertaken, for which genetic markers are needed.<h4>Methods and results</h4>High-throughput sequencing of DNA enriched for microsatellites was used to develop 24 loci that were characterized in <i>E. nuttallii</i>, 21 of which were polymorphic, with the number of alleles ra ...[more]

PMID: 30131888

Similar Datasets

Project description:Biological invasions, especially invasive alien aquatic plants, are a major and growing ecological and socioeconomic problem worldwide. Freshwater systems are particularly vulnerable to invasion, where impacts of invasive alien species can damage ecological structure and function. Identifying abiotic and biotic factors that mediate successful invasions is a management priority. Our aim was to determine the environmental correlates of Elodea nuttallii; a globally significant invasive aquatic species. Elodea nuttallii presence/absence (occurrence), extent (patch area) and percentage cover (density) was visually assessed from a boat throughout Lough Erne (approximately 144 km2), County Fermanagh, Northern Ireland during the active summer growth season (July-September). In addition, substrate type and zebra mussel Dreissena polymorpha occurrence was recorded. Fourteen water chemistry variables were collected monthly from 12 recording stations throughout the lake during the 9 years before the survey to spatially interpolate values and establish temporal trajectories in their change. Shoreline land use was derived from CORINE land cover maps. Environmental associations between E. nuttallii, substrate, D. polymorpha, water chemistry and land use were assessed. Elodea nuttallii occurrence was positively associated with water conductivity, alkalinity, suspended solids, phosphorus (both total and soluble) and chlorophyll-a concentrations, but negatively associated with pH and total oxidised nitrogen. E. nuttallii patch extent and proportional cover were positively associated, to varying degrees, with the presence of D. polymorpha, biological oxygen demand, water clarity and soft substrate, but negatively associated with urban development and ammonium. Elodea nuttallii displayed high levels of phenotypic plasticity in response to environmental variation, allowing it to adapt to a wide range of conditions and potentially gain competitive advantage over native or other invasive macrophytes.It is evident that multiple abiotic and biotic factors, including facilitation by co-occurring invasive dreissenid mussels, interact to influence the distribution and abundance of E. nuttallii. Thus, it is necessary to consider a more comprehensive environmental context when planning Elodea management strategies.