Ontology highlight
ABSTRACT:
SUBMITTER: Liu T
PROVIDER: S-EPMC5948186 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
3 Biotech 20180511 5
One of the most representative core gene sequence of <i>Araneus ventricosus</i> dragline silk protein partial cDNA monomer (JN857964.2) was selected and multimerized using a "head-to-tail" strategy by compatible but nonregenerable sites at both ends resulting in a concatemer of 16 contiguous monomers. This concatemer was cloned into pET-28a(+) expression vector and transformed into <i>Escherichia coli</i>. A 52.6 kDa silk protein was successfully expressed and detected by SDS-PAGE and confirmed ...[more]