Dataset Information

Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte.

ABSTRACT: The use of dsRNA to control insect pests via the RNA interference (RNAi) pathway is being explored by researchers globally. However, with every new class of insect control compounds, the evolution of insect resistance needs to be considered, and understanding resistance mechanisms is essential in designing durable technologies and effective resistance management strategies. To gain insight into insect resistance to dsRNA, a field screen with subsequent laboratory selection was used to establish a population of DvSnf7 dsRNA-resistant western corn rootworm, Diabrotica virgifera virgifera, a major maize insect pest. WCR resistant to ingested DvSnf7 dsRNA had impaired luminal uptake and resistance was not DvSnf7 dsRNA-specific, as indicated by cross resistance to all other dsRNAs tested. No resistance to the Bacillus thuringiensis Cry3Bb1 protein was observed. DvSnf7 dsRNA resistance was inherited recessively, located on a single locus, and autosomal. Together these findings will provide insights for dsRNA deployment for insect pest control.

SUBMITTER: Khajuria C

PROVIDER: S-EPMC5951553 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte.

Khajuria Chitvan C Ivashuta Sergey S Wiggins Elizabeth E Flagel Lex L Moar William W Pleau Michael M Miller Kaylee K Zhang Yuanji Y Ramaseshadri Parthasarathy P Jiang Changjian C Hodge Tracey T Jensen Peter P Chen Mao M Gowda Anilkumar A McNulty Brian B Vazquez Cara C Bolognesi Renata R Haas Jeffrey J Head Graham G Clark Thomas T

PloS one 20180514 5

The use of dsRNA to control insect pests via the RNA interference (RNAi) pathway is being explored by researchers globally. However, with every new class of insect control compounds, the evolution of insect resistance needs to be considered, and understanding resistance mechanisms is essential in designing durable technologies and effective resistance management strategies. To gain insight into insect resistance to dsRNA, a field screen with subsequent laboratory selection was used to establish ...[more]

PMID: 29758046

Similar Datasets

Project description:The western corn rootworm, Diabrotica virgifera virgifera (LeConte) (Coleoptera: Chrysomelidae), is an economically important pest of corn in the northern United States. Some populations have developed resistance to management strategies including transgenic corn that produces insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt). Knowledge of insect dispersal is of critical importance for models of resistance evolution. Larval density affects survival in the field, and stress from crowding often affects facultative long-distance dispersal of adult insects. In this study, we used laboratory flight mills to characterize western corn rootworm flight performance as a function of larval rearing density. Larvae were reared under three densities and the resulting adult females were either allowed to fly voluntarily for 22 h or forced to fly specified durations. For both experiments we also measured lifetime fecundity following flight. The three rearing densities placed differential levels of stress on individuals, as evidenced by decreased survival to adulthood and decreased size of adults at greater rearing density. When larvae were reared under crowded conditions the resulting females were more likely to engage in flight activity, including long uninterrupted flights lasting >10 min, than those reared under low density conditions. Flight and egg production are both energy intensive processes. However, we found no evidence in either voluntary or forced flight experiments of a tradeoff between flight activity and female fecundity. The results suggest that females emerging from high density populations in cornfields are more likely to disperse and disperse farther than those emerging from low density populations. These results are important because they imply that variation in population density in the landscape will affect dispersal, which may in turn require computer models of resistance evolution to incorporate multiple dispersal rates arising from varying larval densities among fields.

Dataset Information

Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte.

Publications

Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets