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The Carotenogenic Dunaliella salina CCAP 19/20 Produces Enhanced Levels of Carotenoid under Specific Nutrients Limitation.


ABSTRACT: Dunaliella salina is the popular microalga for ?-carotene production. There is still a growing demand for the best strain identification and growth conditions optimization for maximum carotenoids production. Some strains are noncarotenogenic while other strains may respond differently to applied growth conditions and produce enhanced carotenoid levels. This study tested the carotenogenic ability of Dunaliella salina CCAP 19/20 under sixteen stress conditions and certain biochemical changes in response to specific stress were investigated. This study identified the above strain as carotenogenic, which produces maximum carotenoids under high light (240??mol photons m-2?sec-1) when combined nitrogen and micronutrients (Cu or CuMn) were limited. Based on the intensity of extracted ions chromatograms, lutein (m/z 568.4357) appears as the major carotenoid followed by ?-carotene (m/z 536.4446) and ?-carotene (m/z 536.4435). A polypeptide of 28.3?kDa appeared while another polypeptide of 25.5?kDa disappeared in stress cells as compared to noncarotenogenic cells. Expression levels of antioxidative-enzyme superoxide dismutase-1 (SOD1, H2O2-resistant) remained identical, while the prominent H2O2-sensitive isoforms SOD2 and SOD3 were downregulated during carotenogenic conditions. Overall, increased carotenoids levels might be due to the response of differential expression of specific polypeptides and retention of H2O2-resistant SOD, which eventually might help the organism to thrive in the tested stress conditions.

SUBMITTER: Saha SK 

PROVIDER: S-EPMC5952566 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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The Carotenogenic <i>Dunaliella salina</i> CCAP 19/20 Produces Enhanced Levels of Carotenoid under Specific Nutrients Limitation.

Saha Sushanta Kumar SK   Kazipet Naresh N   Murray Patrick P  

BioMed research international 20180430


<i>Dunaliella salina</i> is the popular microalga for <i>β</i>-carotene production. There is still a growing demand for the best strain identification and growth conditions optimization for maximum carotenoids production. Some strains are noncarotenogenic while other strains may respond differently to applied growth conditions and produce enhanced carotenoid levels. This study tested the carotenogenic ability of <i>Dunaliella salina</i> CCAP 19/20 under sixteen stress conditions and certain bioc  ...[more]

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