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Disease-induced assemblage of a plant-beneficial bacterial consortium.

ABSTRACT: Disease suppressive soils typically develop after a disease outbreak due to the subsequent assembly of protective microbiota in the rhizosphere. The role of the plant immune system in the assemblage of a protective rhizosphere microbiome is largely unknown. In this study, we demonstrate that Arabidopsis thaliana specifically promotes three bacterial species in the rhizosphere upon foliar defense activation by the downy mildew pathogen Hyaloperonospora arabidopsidis. The promoted bacteria were isolated and found to interact synergistically in biofilm formation in vitro. Although separately these bacteria did not affect the plant significantly, together they induced systemic resistance against downy mildew and promoted growth of the plant. Moreover, we show that the soil-mediated legacy of a primary population of downy mildew infected plants confers enhanced protection against this pathogen in a second population of plants growing in the same soil. Together our results indicate that plants can adjust their root microbiome upon pathogen infection and specifically recruit a group of disease resistance-inducing and growth-promoting beneficial microbes, therewith potentially maximizing the chance of survival of their offspring that will grow in the same soil.

SUBMITTER: Berendsen RL

PROVIDER: S-EPMC5956071 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Disease-induced assemblage of a plant-beneficial bacterial consortium.

Berendsen Roeland L RL Vismans Gilles G Yu Ke K Song Yang Y de Jonge Ronnie R Burgman Wilco P WP Burmølle Mette M Herschend Jakob J Bakker Peter A H M PAHM Pieterse Corné M J CMJ

The ISME journal 20180308 6

Disease suppressive soils typically develop after a disease outbreak due to the subsequent assembly of protective microbiota in the rhizosphere. The role of the plant immune system in the assemblage of a protective rhizosphere microbiome is largely unknown. In this study, we demonstrate that Arabidopsis thaliana specifically promotes three bacterial species in the rhizosphere upon foliar defense activation by the downy mildew pathogen Hyaloperonospora arabidopsidis. The promoted bacteria were is ...[more]

PMID: 29520025

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Project description:The beneficial effect of microbial consortium application on plants is strongly affected by soil conditions, which are influenced by farming practices. The establishment of microbial inoculants in the rhizosphere is a prerequisite for successful plant-microorganism interactions. This study investigated whether a consortium of beneficial microorganisms establishes in the rhizosphere of a winter crop during the vegetation period, including the winter growing season. In addition, we aimed for a better understanding of its effect on plant performance under different farming practices. Winter rye plants grown in a long-time field trial under conventional or organic farming practices were inoculated after plant emergence in autumn with a microbial consortium containing Pseudomonas sp. (RU47), Bacillus atrophaeus (ABi03) and Trichoderma harzianum (OMG16). The density of the microbial inoculants in the rhizosphere and root-associated soil was quantified in autumn and the following spring. Furthermore, the influence of the consortium on plant performance and on the rhizosphere bacterial community assembly was investigated using a multidisciplinary approach. Selective plating showed a high colonization density of individual microorganisms of the consortium in the rhizosphere and root-associated soil of winter rye throughout its early growth cycle. 16S rRNA gene amplicon sequencing showed that the farming practice affected mainly the rhizosphere bacterial communities in autumn and spring. However, the microbial consortium inoculated altered also the bacterial community composition at each sampling time point, especially at the beginning of the new growing season in spring. Inoculation of winter rye with the microbial consortium significantly improved the plant nutrient status and performance especially under organic farming. In summary, the microbial consortium showed sufficient efficacy throughout vegetation dormancy when inoculated in autumn and contributed to better plant performance, indicating the potential of microbe-based solutions in organic farming where nutrient availability is limited.

Project description:Many effective plant-microbe interactions lead to biological changes that can stimulate plant growth and production. This study evaluated the effect of the interaction between quinoa (Chenopodium quinoa Willd.) and endophytic bacterial strains on differential responses under biotic stress. Four strains of endophytic bacteria were used to inoculate three quinoa genotypes. Endophytic bacteria, isolated from the endosphere of healthy genotypes of quinoa plants, were used to evaluate their biocontrol activity against Pseudomonas syringae on quinoa plants, which causes leaf spot disease, depending on some different parameters. Quinoa genotype plants were treated with four treatments: pathogenic bacteria only (T1), internal bacteria only (T2), pathogenic bacteria + endogenous bacteria (T3), and untreated as the control (T4). The results indicated that there was a significant difference between chlorophyll content index of infected plants without bioagent (untreated) compared to plants bio-inoculated with endophytic bacteria. The highest mean disease incidence was on the plants without bacterial inoculum (90, 80, and 100%) for quinoa genotypes G1, G2, and G3, respectively. The results showed that there were significant differences in the weight of grains/plant, as the value ranged from 8.1 to 13.3 g when treated with pathogens (T1) compared to the treatment with pathogens and endogenous bacteria (T3), which ranged from 11.7 to 18.6 g/plant. Decreases in total aromatic amino acids appeared due to the pathogen infection, by 6.3, 22.8, and 24.1% (compared to the control) in G1, G2, and G3, respectively. On the other hand, genotype G3 showed the highest response in the levels of total aromatic and total neutral amino acids. The endophytic strains promoted quinoa seedling growth mainly by improving nutrient efficiency. This improvement could not be explained by their ability to induce the production of amino acids, showing that complex interactions might be associated with enhancement of quinoa seedling performance by endophytic bacteria. The endophytic bacterial strains were able to reduce the severity of bacterial leaf spot disease by 30, 40, and 50% in quinoa genotypes G1, G2, and G3, respectively, recording significant differences compared to the negative control. The results indicated that, G1 genotype was superior in different performance indicators (pathogen tolerance index, yield injury %, superiority measure and relative performance) for grain weight/plant under pathogen infection condition when treated with endophyte bacteria. Based on this study, these bacterial strains can be used as a biotechnology tool in quinoa seedling production and biocontrol to diminish the severity of bacterial leaf spot disease.

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Dataset Information

Disease-induced assemblage of a plant-beneficial bacterial consortium.

Publications

Disease-induced assemblage of a plant-beneficial bacterial consortium.

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