Dataset Information

PTRA - A reporter system for monitoring the intracellular dynamics of gene expression.

ABSTRACT: The presence of standardised tools and methods to measure and represent accurately biological parts and functions is a prerequisite for successful metabolic engineering and crucial to understand and predict the behaviour of synthetic genetic circuits. Many synthetic gene networks are based on transcriptional circuits, thus information on transcriptional and translational activity is important for understanding and fine-tuning the synthetic function. To this end, we have developed a toolkit to analyse systematically the transcriptional and translational activity of a specific synthetic part in vivo. It is based on the plasmid pTRA and allows the assignment of specific transcriptional and translational outputs to the gene(s) of interest (GOI) and to compare different genetic setups. By this, the optimal combination of transcriptional strength and translational activity can be identified. The design is tested in a case study using the gene encoding the fluorescent mCherry protein as GOI. We show the intracellular dynamics of mRNA and protein formation and discuss the potential and shortcomings of the pTRA plasmid.

SUBMITTER: Wagner SG

PROVIDER: S-EPMC5957375 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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pTRA - A reporter system for monitoring the intracellular dynamics of gene expression.

Wagner Sabine G SG Ziegler Martin M Löwe Hannes H Kremling Andreas A Pflüger-Grau Katharina K

PloS one 20180517 5

The presence of standardised tools and methods to measure and represent accurately biological parts and functions is a prerequisite for successful metabolic engineering and crucial to understand and predict the behaviour of synthetic genetic circuits. Many synthetic gene networks are based on transcriptional circuits, thus information on transcriptional and translational activity is important for understanding and fine-tuning the synthetic function. To this end, we have developed a toolkit to an ...[more]

PMID: 29772009

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Dataset Information

PTRA - A reporter system for monitoring the intracellular dynamics of gene expression.

Publications

pTRA - A reporter system for monitoring the intracellular dynamics of gene expression.

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