Supernatant protein biomarkers of red blood cell storage hemolysis as determined through an absolute quantification proteomics technology.

ABSTRACT:

Background

Laboratory technologies have highlighted the progressive accumulation of the so-called "storage lesion," a wide series of alterations to stored red blood cells (RBCs) that may affect the safety and effectiveness of the transfusion therapy. New improvements in the field are awaited to ameliorate this lesion, such as the introduction of washing technologies in the cell processing pipeline. Laboratory studies that have tested such technologies so far rely on observational qualitative or semiquantitative techniques.

Study design and methods

A state-of-the-art quantitative proteomics approach utilizing quantitative concatamers (QconCAT) was used to simultaneously monitor fluctuations in the abundance of 114 proteins in AS-3 RBC supernatants (n = 5; 11 time points, incl

SUBMITTER: D'Alessandro A 

PROVIDER: S-EPMC5957548 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature