Spectrally Resolved Fiber Photometry for Multi-component Analysis of Brain Circuits.

ABSTRACT: To achieve simultaneous measurement of multiple cellular events in molecularly defined groups of neurons in vivo, we designed a spectrometer-based fiber photometry system that allows for spectral unmixing of multiple fluorescence signals recorded from deep brain structures in behaving animals. Using green and red Ca²⁺ indicators differentially expressed in striatal direct- and indirect-pathway neurons, we were able to simultaneously monitor the neural activity in these two pathways in freely moving animals. We found that the activities were highly synchronized between the direct and indirect pathways within one hemisphere and were desynchronized between the two hemispheres. We further analyzed the relationship between the movement patterns and the magnitude of activation in dire

SUBMITTER: Meng C 

PROVIDER: S-EPMC5957785 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature