Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags.

ABSTRACT: Upon expression of a given protein in an expression host, its secretion into the culture medium or cell-surface display is frequently advantageous in both research and industrial contexts. Hence, engineering strategies targeting folding, trafficking, and secretion of the proteins gain considerable interest. Yarrowia lipolytica has emerged as an efficient protein expression platform, repeatedly proved to be a competitive secretor of proteins. Although the key role of signal peptides (SPs) in secretory overexpression of proteins and their direct effect on the final protein titers are widely known, the number of reports on manipulation with SPs in Y. lipolytica is rather scattered. In this study, we assessed the potential of ten different SPs for secretion of two heterologous proteins in Y. l

SUBMITTER: Celinska E 

PROVIDER: S-EPMC5959983 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature