Fully electronic urine dipstick probe for combinatorial detection of inflammatory biomarkers.

ABSTRACT: Aim:An electrochemical urine dipstick probe biosensor has been demonstrated using molybdenum electrodes on nanoporous polyamide substrate for the quantitative detection of two inflammatory protein biomarkers, CRP and IL-6. Materials & methods:The electrode interface was characterized using ?-potential and Fourier transform infrared spectroscopy. Detection of biomarkers was demonstrated by measuring impedance changes associated with the dose concentrations of the two biomarkers. A proof of feasibility of point-of-care implementation of the biosensor was demonstrated using a portable electronics platform. Results & conclusion:Limit of detection of 1 pg/ml was achieved for CRP and IL-6 in human urine and synthetic urine buffers. The developed portable hardware demonstrated close correlation with benchtop equipment results.

SUBMITTER: Kamakoti V 

PROVIDER: S-EPMC5961415 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature