PolNet: A Tool to Quantify Network-Level Cell Polarity and Blood Flow in Vascular Remodeling.
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ABSTRACT:
SUBMITTER: Bernabeu MO
PROVIDER: S-EPMC5961748 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Publications
PolNet: A Tool to Quantify Network-Level Cell Polarity and Blood Flow in Vascular Remodeling.
Bernabeu Miguel O MO Jones Martin L ML Nash Rupert W RW Pezzarossa Anna A Coveney Peter V PV Gerhardt Holger H Franco Claudio A CA
Biophysical journal 20180501 9
In this article, we present PolNet, an open-source software tool for the study of blood flow and cell-level biological activity during vessel morphogenesis. We provide an image acquisition, segmentation, and analysis protocol to quantify endothelial cell polarity in entire in vivo vascular networks. In combination, we use computational fluid dynamics to characterize the hemodynamics of the vascular networks under study. The tool enables, to our knowledge for the first time, a network-level analy ...[more]
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