An Oil-Free Picodrop Bioassay Platform for Synthetic Biology.
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ABSTRACT:
SUBMITTER: Siltanen CA
PROVIDER: S-EPMC5962535 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Publications
An Oil-Free Picodrop Bioassay Platform for Synthetic Biology.
Siltanen Christian A CA Cole Russell H RH Poust Sean S Chao Lawrence L Tyerman Jabus J Kaufmann-Malaga Benjamin B Ubersax Jeff J Gartner Zev J ZJ Abate Adam R AR
Scientific reports 20180521 1
Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Microfluidics to construct defined reactions with chemicals and cells incubated under air on an open array. The method interfaces with most bioanalytical tools and retains hydrophobic compounds in compar ...[more]
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