Extremely low frequency electromagnetic fields promote mesenchymal stem cell migration by increasing intracellular Ca²⁺ and activating the FAK/Rho GTPases signaling pathways in vitro.

ABSTRACT:

Background

The ability of mesenchymal stem cells (MSCs) to migrate to the desired tissues or lesions is crucial for stem cell-based regenerative medicine and tissue engineering. Optimal therapeutics for promoting MSC migration are expected to become an effective means for tissue regeneration. Electromagnetic fields (EMF), as a noninvasive therapy, can cause a lot of biological changes in MSCs. However, whether EMF can promote MSC migration has not yet been reported.

Methods

We evaluated the effects of EMF on cell migration in human bone marrow-derived MSCs. With the use of Helmholtz coils and an EMF stimulator, 7.5, 15, 30, 50, and 70 Hz/1 mT EMF was generated. Additionally, we employed the L-type calcium channel blocker verapamil and the focal adhesion kinase (FAK) inhibito

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5963142 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature