Dataset Information

Dysregulation of miR-200s clusters as potential prognostic biomarkers in acute myeloid leukemia.

ABSTRACT:

Background

Increasing studies showed that miR-200 family (miR-200s) clusters are aberrantly expressed in multiple human cancers, and miR-200s clusters function as tumor suppressor genes by affecting cell proliferation, self-renewal, differentiation, division and apoptosis. Herein, we aimed to investigate the expression and clinical implication of miR-200s clusters in acute myeloid leukemia (AML).

Methods

RT-qPCR was performed to detect expression of miR-200s clusters in 19 healthy donors, 98 newly diagnosed AML patients, and 35 AML patients achieved complete remission (CR).

Results

Expression of miR-200a/200b/429 cluster but not miR-200c/141 cluster was decreased in newly diagnosed AML patients as compared to healthy donors and AML patients achieved CR. Although no significant differences were observed between miR-200s clusters and most of the features, low expression of miR-200s clusters seems to be associated with higher white blood cells especially for miR-200a/200b. Of the five members of miR-200s clusters, low expression of miR-200b/429/200c was found to be associated with lower CR rate. Logistic regression analysis further revealed that low expression of miR-429 acted as an independent risk factor for CR in AML. Based on Kaplan-Meier analysis, low expression of miR-200b/429/200c was associated with shorter OS, whereas miR-200a/141 had a trend. Moreover, multivariate analysis of Cox regression models confirmed the independently prognostic value of miR-200b expression for OS in AML.

Conclusions

Expression of miR-200a/200b/429 cluster was frequently down-regulated in AML, and low expression of miR-429 as an independent risk factor for CR, whereas low expression of miR-200b as an independent prognostic biomarker for OS.

SUBMITTER: Zhou JD

PROVIDER: S-EPMC5963159 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Publications

Dysregulation of miR-200s clusters as potential prognostic biomarkers in acute myeloid leukemia.

Zhou Jing-Dong JD Zhou Jing-Dong JD Zhang Liu-Chao LC Zhang Ting-Juan TJ Gu Yu Y Wu De-Hong DH Zhang Wei W Ma Ji-Chun JC Wen Xiang-Mei XM Guo Hong H Lin Jiang J Qian Jun J

Journal of translational medicine 20180521 1

<h4>Background</h4>Increasing studies showed that miR-200 family (miR-200s) clusters are aberrantly expressed in multiple human cancers, and miR-200s clusters function as tumor suppressor genes by affecting cell proliferation, self-renewal, differentiation, division and apoptosis. Herein, we aimed to investigate the expression and clinical implication of miR-200s clusters in acute myeloid leukemia (AML).<h4>Methods</h4>RT-qPCR was performed to detect expression of miR-200s clusters in 19 healthy ...[more]

PMID: 29784043

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Project description:BACKGROUND:microRNA-381 is dysregulated in a variety of cancers. However, its clinical significance in pediatric acute myeloid leukemia (AML) is still unclear. The purpose of this study was to detect the expression level of miR-381 in pediatric AML patients and to explore its potential clinical significance. METHODS:The levels of miR-381 in bone marrow and serum of 102 pediatric AML patients were measured by quantitative real-time polymorperase chain reaction (qRT-PCR). The diagnostic value of serum miR-381 in pediatric AML patients was evaluated by the receiver operating characteristic (ROC) curve. A Chi square test was used to analyze the relationship between serum miR-381 and the clinical characteristics of patients. Cox regression analysis and Kaplan-Meier evaluated the prognostic value of serum miR-381 in patients. Finally, the proliferation of the cells was analyzed by the CCK-8 assay. RESULTS:Compared with healthy controls, the levels of miR-381 in serum and bone marrow of pediatric AML patients were significantly decreased (P?<?0.001). ROC curve showed that miR-381 could distinguish pediatric AML cases from normal controls. At the same time, the downregulation of miR-381 was associated with M7 in the French-American-British (FAB) classifications and unfavorable cytogenetic risks (P?<?0.05). Low serum miR-381 levels were associated with poor overall survival of pediatric AML (log-rank test, P?=?0.011) and poor relapse-free survival (log-rank test, P?=?0.004). Cox regression analysis confirmed that reduced serum miR-381 was an independent predictor of poor prognosis in AML (HR?=?3.794, 95% CI 1.3633-10.559, P?=?0.011). In addition, low expression of miR-381 significantly reduced the proliferation of cells (P?<?0.05). CONCLUSION:All experimental results confirm that miR-381 has reduced bone marrow and serum expression in pediatric AML, and low levels of serum miR-381 have certain diagnostic and prognostic value in pediatric AML and may be a potential therapeutic target for AML.

Dataset Information

Dysregulation of miR-200s clusters as potential prognostic biomarkers in acute myeloid leukemia.

Background

Methods

Results

Conclusions

Publications

Dysregulation of miR-200s clusters as potential prognostic biomarkers in acute myeloid leukemia.

Similar Datasets

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