Ontology highlight
ABSTRACT:
SUBMITTER: Zhang P
PROVIDER: S-EPMC5964124 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Zhang Peng P Xia Ji-Han JH Zhu Jing J Gao Ping P Tian Yi-Jun YJ Du Meijun M Guo Yong-Chen YC Suleman Sufyan S Zhang Qin Q Kohli Manish M Tillmans Lori S LS Thibodeau Stephen N SN French Amy J AJ Cerhan James R JR Wang Li-Dong LD Wei Gong-Hong GH Wang Liang L
Nature communications 20180522 1
Functional characterization of disease-causing variants at risk loci has been a significant challenge. Here we report a high-throughput single-nucleotide polymorphisms sequencing (SNPs-seq) technology to simultaneously screen hundreds to thousands of SNPs for their allele-dependent protein-binding differences. This technology takes advantage of higher retention rate of protein-bound DNA oligos in protein purification column to quantitatively sequence these SNP-containing oligos. We apply this te ...[more]