Ontology highlight
ABSTRACT:
SUBMITTER: Zhou Y
PROVIDER: S-EPMC5964179 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Zhou Yanwen Y Chen Xinyu X Kang Bo B She Shiqi S Zhang Xiaobing X Chen Cheng C Li Wenxin W Chen Wenjie W Dan Songsong S Pan Xiaoyun X Liu Xiaoli X He Jianqin J Zhao Qingwei Q Zhu Chenggang C Peng Ling L Wang Haoyi H Yao Hangping H Cao Hongcui H Li Lanjuan L Herlyn Meenhard M Wang Ying-Jie YJ
Cell death & disease 20180522 6
OCT4A is well established as a master transcription factor for pluripotent stem cell (PSC) self-renewal and a pioneer factor for initiating somatic cell reprogramming, yet its presence and functionality in somatic cancer cells remain controversial and obscure. By combining the CRISPR-Cas9-based gene editing with highly specific PCR assays, highly sensitive immunoassays, and mass spectrometry, we provide unequivocal evidence here that full-length authentic OCT4A transcripts and proteins were both ...[more]