Unknown

Dataset Information

0

A vaccinia-based single vector construct multi-pathogen vaccine protects against both Zika and chikungunya viruses.


ABSTRACT: Zika and chikungunya viruses have caused major epidemics and are transmitted by Aedes aegypti and/or Aedes albopictus mosquitoes. The "Sementis Copenhagen Vector" (SCV) system is a recently developed vaccinia-based, multiplication-defective, vaccine vector technology that allows manufacture in modified CHO cells. Herein we describe a single-vector construct SCV vaccine that encodes the structural polyprotein cassettes of both Zika and chikungunya viruses from different loci. A single vaccination of mice induces neutralizing antibodies to both viruses in wild-type and IFNAR-/- mice and protects against (i) chikungunya virus viremia and arthritis in wild-type mice, (ii) Zika virus viremia and fetal/placental infection in female IFNAR-/- mice, and (iii) Zika virus viremia and testes infection and pathology in male IFNAR-/- mice. To our knowledge this represents the first single-vector construct, multi-pathogen vaccine encoding large polyproteins, and offers both simplified manufacturing and formulation, and reduced "shot burden" for these often co-circulating arboviruses.

SUBMITTER: Prow NA 

PROVIDER: S-EPMC5964325 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications


Zika and chikungunya viruses have caused major epidemics and are transmitted by Aedes aegypti and/or Aedes albopictus mosquitoes. The "Sementis Copenhagen Vector" (SCV) system is a recently developed vaccinia-based, multiplication-defective, vaccine vector technology that allows manufacture in modified CHO cells. Herein we describe a single-vector construct SCV vaccine that encodes the structural polyprotein cassettes of both Zika and chikungunya viruses from different loci. A single vaccination  ...[more]

Similar Datasets

| S-EPMC7265471 | biostudies-literature
| S-EPMC1424664 | biostudies-literature
| S-EPMC7738162 | biostudies-literature
| S-EPMC6255889 | biostudies-literature
| S-EPMC6343128 | biostudies-literature
| S-EPMC5526527 | biostudies-literature
| S-EPMC7941830 | biostudies-literature
| S-EPMC7100368 | biostudies-literature
| S-EPMC8707647 | biostudies-literature
| S-EPMC6466193 | biostudies-literature