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Aqueous Humor Dynamics of the Brown-Norway Rat.


ABSTRACT: Purpose:The study aimed to provide a quantitative description of aqueous humor dynamics in healthy rat eyes. Methods:One eye of 26 anesthetized adult Brown-Norway rats was cannulated with a needle connected to a perfusion pump and pressure transducer. Pressure-flow data were measured in live and dead eyes by varying pump rate (constant-flow technique) or by modulating pump duty cycle to hold intraocular pressure (IOP) at set levels (modified constant-pressure technique). Data were fit by the Goldmann equation to estimate conventional outflow facility (C) and unconventional outflow rate (Fun). Parameter estimates were respectively checked by inserting a shunt of similar conductance into the eye and by varying eye hydration methodology. Results:Rat IOP averaged 14.6 ± 1.9 mm Hg at rest. Pressure-flow data were repeatable and indistinguishable for the two perfusion techniques, yielding C = 0.023 ± 0.002 ?L/min/mm Hg and Fun = 0.096 ± 0.024 ?L/min. C was similar for live and dead eyes and increased upon shunt insertion by an amount equal to shunt conductance, validating measurement accuracy. At 100% humidity Fun dropped to 0.003 ± 0.030 ?L/min. Physiological washout was not observed (-0.35 ± 0.65%/h), and trabecular anatomy looked normal. Conclusions:Rat aqueous humor dynamics are intermediate in magnitude compared to those in mice and humans, consistent with species differences in eye size. C does not change with time or death. Evaporation complicates measurement of Fun even when eyes are not enucleated. Absence of washout is a notable finding seen only in mouse and human eyes to date.

SUBMITTER: Ficarrotta KR 

PROVIDER: S-EPMC5967599 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Aqueous Humor Dynamics of the Brown-Norway Rat.

Ficarrotta Kayla R KR   Bello Simon A SA   Mohamed Youssef H YH   Passaglia Christopher L CL  

Investigative ophthalmology & visual science 20180501 6


<h4>Purpose</h4>The study aimed to provide a quantitative description of aqueous humor dynamics in healthy rat eyes.<h4>Methods</h4>One eye of 26 anesthetized adult Brown-Norway rats was cannulated with a needle connected to a perfusion pump and pressure transducer. Pressure-flow data were measured in live and dead eyes by varying pump rate (constant-flow technique) or by modulating pump duty cycle to hold intraocular pressure (IOP) at set levels (modified constant-pressure technique). Data were  ...[more]

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