Structure-based analysis of CysZ-mediated cellular uptake of sulfate.

ABSTRACT: Sulfur, most abundantly found in the environment as sulfate (SO₄^2-), is an essential element in metabolites required by all living cells, including amino acids, co-factors and vitamins. However, current understanding of the cellular delivery of SO₄^2- at the molecular level is limited. CysZ has been described as a SO₄^2- permease, but its sequence family is without known structural precedent. Based on crystallographic structure information, SO₄^2- binding and flux experiments, we provide insight into the molecular mechanism of CysZ-mediated translocation of SO₄^2- across membranes. CysZ structures from three different bacterial species display a hitherto unknown fold and have subunits

SUBMITTER: Assur Sanghai Z 

PROVIDER: S-EPMC5967866 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature