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Carbohydrate microarrays for screening functional glycans.


ABSTRACT: Carbohydrate microarrays have become robust and powerful tools for the rapid analysis of glycan-associated binding events. However, this microarray technology has rarely been applied in studies of glycan-mediated cellular responses. Herein we describe a carbohydrate microarray-based approach for the rapid screening of biologically active glycans that stimulate the production of reactive oxygen species (ROS) through binding to the cell-surface lectin. We employed a microarray assay and a fluorescent ROS probe to identify the functional glycans which enhance ROS production. Cells binding to glycans on the microarrays produced ROS, whose levels were decreased in the presence of a ROS scavenger or a NADPH oxidase inhibitor. The present study leads us to suggest that glycan microarrays are applicable to the simultaneous screening of various glycans whose binding to the cell-surface lectin elicits cellular response.

SUBMITTER: Pai J 

PROVIDER: S-EPMC5968531 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Carbohydrate microarrays for screening functional glycans.

Pai Jaeyoung J   Hyun Ji Young JY   Jeong Jieun J   Loh Sohee S   Cho Eun-Hee EH   Kang Young-Sun YS   Shin Injae I  

Chemical science 20160105 3


Carbohydrate microarrays have become robust and powerful tools for the rapid analysis of glycan-associated binding events. However, this microarray technology has rarely been applied in studies of glycan-mediated cellular responses. Herein we describe a carbohydrate microarray-based approach for the rapid screening of biologically active glycans that stimulate the production of reactive oxygen species (ROS) through binding to the cell-surface lectin. We employed a microarray assay and a fluoresc  ...[more]

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