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A smart "off-on" gate for the in situ detection of hydrogen sulphide with Cu(ii)-assisted europium emission.

ABSTRACT: A water-soluble and emissive Eu-complex (EuL1) bearing a DO3A(Eu3+)-pyridine-aza-crown motif has been prepared and its Cu2+ complex has been demonstrated to be a smart luminescence "off-on" gate for H2S detection in water with a nano-molar detection limit (60 nM). EuL1 binds to Cu2+ ions selectively (KB = 1.2 × 105 M-1) inducing 17-fold luminescence quenching and forming a 1?:?1 stoichiometric complex (EuL1-Cu2+), which responds to H2S selectively with restoration of the original Eu emission of EuL1 followed by a further 40-fold luminescence enhancement, forming a 1?:?1 stoichiometric complex (EuL1-Na2S, KB = 1.5 × 104 M-1). Without Cu2+ ions, EuL1 showed non-specific binding towards H2S with only a 5-fold luminescence enhancement.

SUBMITTER: Liang Z

PROVIDER: S-EPMC5968757 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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A smart "off-on" gate for the in situ detection of hydrogen sulphide with Cu(ii)-assisted europium emission.

Liang Zhenhao Z Tsoi Tik-Hung TH Chan Chi-Fai CF Dai Lixiong L Wu Yudan Y Du Guangyan G Zhu Lizhi L Lee Chi-Sing CS Wong Wing-Tak WT Law Ga-Lai GL Wong Ka-Leung KL

Chemical science 20151207 3

A water-soluble and emissive Eu-complex (EuL1) bearing a DO3A(Eu3+)-pyridine-aza-crown motif has been prepared and its Cu2+ complex has been demonstrated to be a smart luminescence "off-on" gate for H2S detection in water with a nano-molar detection limit (60 nM). EuL1 binds to Cu2+ ions selectively (KB = 1.2 × 105 M-1) inducing 17-fold luminescence quenching and forming a 1 : 1 stoichiometric compl ...[more]

PMID: 29899943

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Project description:IntroductionHydrogen sulphide (H2S) has been established as a key member of the gasotransmitters family that recently showed a pivotal role in various pathological conditions including cancer.ObjectivesThis study investigated the role of H2S in breast cancer (BC) pathogenesis, on BC immune recognition capacity and the consequence of targeting H2S using non-coding RNAs.MethodsEighty BC patients have been recruited for the study. BC cell lines were cultured and transfected using validated oligonucleotide delivery system. Gene and protein expression analysis was performed using qRT-PCR, western blot and flow-cytometry. In-vitro analysis for BC hallmarks was performed using MTT, BrdU, Modified Boyden chamber, migration and colony forming assays. H2S and nitric oxide (NO) levels were measured spectrophotometrically. Primary natural killer cells (NK cells) and T cell isolation and chimeric antigen receptor transduction (CAR T cells) were performed using appropriate kits. NK and T cells cytotoxicity was measured. Finally, computational target prediction analysis and binding confirmation analyses were performed using different software and dual luciferase assay kit, respectively.ResultsThe H2S synthesizing enzymes, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE), exhibited elevated levels in the clinical samples that correlated with tumor proliferation index. Knock-down of CBS and CSE in the HER2+ BC and triple negative BC (TNBC) cells resulted in significant attenuation of BC malignancy. In addition to increased susceptibility of HER2+ BC and TNBC to the cytotoxic activity of HER2 targeting CAR T cells and NK cells, respectively. Transcriptomic and phosphoprotein analysis revealed that H2S signaling is mediated through Akt in MCF7, STAT3 in MDA-MB-231 and miR-155/ NOS2/NO signaling in both cell lines. Lastly, miR-4317 was found to function as an upstream regulator of CBS and CSE synergistically abrogates the malignancy of BC cells.ConclusionThese findings demonstrate the potential role of H2S signaling in BC pathogenesis and the potential of its targeting for disease mitigation.

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A smart "off-on" gate for the in situ detection of hydrogen sulphide with Cu(ii)-assisted europium emission.

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A smart "off-on" gate for the <i>in situ</i> detection of hydrogen sulphide with Cu(ii)-assisted europium emission.

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