The CaMKII/NMDA receptor complex controls hippocampal synaptic transmission by kinase-dependent and independent mechanisms.

ABSTRACT: CaMKII is one of the most studied synaptic proteins, but many critical issues regarding its role in synaptic function remain unresolved. Using a CRISPR-based system to delete CaMKII and replace it with mutated forms in single neurons, we have rigorously addressed its various synaptic roles. In brief, basal AMPAR and NMDAR synaptic transmission both require CaMKII?, but not CaMKII?, indicating that, even in the adult, synaptic transmission is determined by the ongoing action of CaMKII?. While AMPAR transmission requires kinase activity, NMDAR transmission does not, implying a scaffolding role for the CaMKII protein instead. LTP is abolished in the absence of CaMKII? and/or CaMKII? and with an autophosphorylation impaired CaMKII? (T286A). With the exception of NMDAR synaptic currents, all aspects of CaMKII? signaling examined require binding to the NMDAR, emphasizing the essential role of this receptor as a master synaptic signaling hub.

SUBMITTER: Incontro S 

PROVIDER: S-EPMC5970233 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature