Ontology highlight
ABSTRACT:
SUBMITTER: Rullan M
PROVIDER: S-EPMC5971206 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Rullan Marc M Benzinger Dirk D Schmidt Gregor W GW Milias-Argeitis Andreas A Khammash Mustafa M
Molecular cell 20180501 4
Transcription is a highly regulated and inherently stochastic process. The complexity of signal transduction and gene regulation makes it challenging to analyze how the dynamic activity of transcriptional regulators affects stochastic transcription. By combining a fast-acting, photo-regulatable transcription factor with nascent RNA quantification in live cells and an experimental setup for precise spatiotemporal delivery of light inputs, we constructed a platform for the real-time, single-cell i ...[more]