Ontology highlight
ABSTRACT:
SUBMITTER: Higashimoto Y
PROVIDER: S-EPMC5971533 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Higashimoto Yuki Y Ihira Masaru M Miyazaki Yu Y Kuboshiki Ayumi A Yoshinaga Sayaka S Hiramatsu Hiroyuki H Suzuki Ryota R Miyata Masafumi M Miura Hiroki H Komoto Satoshi S Yukitake Jun J Taniguchi Koki K Kawamura Yoshiki Y Yoshikawa Tetsushi T
Journal of clinical microbiology 20180525 6
RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific real-time reverse transcription-PCR (RT-PCR) assays. Five real-time RT-PCR assays were designed for the VP7 gene in genotypes G1, G2, G3, G4, and G6. All assays exhibited excellent li ...[more]