Ontology highlight
ABSTRACT:
SUBMITTER: Petell CJ
PROVIDER: S-EPMC5972016 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Petell Christopher J CJ Loiseau Gilbert G Gandy Ryan R Pradhan Sriharsa S Gowher Humaira H
Analytical biochemistry 20170615
DNA methylation is a highly conserved epigenetic modification with critical roles ranging from protection against phage infection in bacteria to the regulation of gene expression in mammals. DNA methylation at specific sequences can be measured by using methylation dependent or sensitive restriction enzymes coupled to semi- or quantitative PCR (MD-qPCR). This study reports a refined MD-qPCR method for detecting gain or loss of DNA methylation at specific sites through the specific use of MspJI o ...[more]