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Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion.


ABSTRACT: Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A•T to G•C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants. An endogenous gene is also successfully modified through introducing a gain-of-function point mutation to directly produce an herbicide-tolerant rice plant. With this new adenine base editing system, it is now possible to precisely edit all base pairs, thus expanding the toolset for precise editing in plants.

SUBMITTER: Li C 

PROVIDER: S-EPMC5972399 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion.

Li Chao C   Zong Yuan Y   Wang Yanpeng Y   Jin Shuai S   Zhang Dingbo D   Song Qianna Q   Zhang Rui R   Gao Caixia C  

Genome biology 20180529 1


Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A•T to G•C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants. An endogenous gene is also successfully modified through introducing a gain-of-function point mutation to directly produce an herbicide-tolerant rice plant. With  ...[more]

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