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Conformation and dynamics of soluble repetitive domain elucidates the initial ?-sheet formation of spider silk.


ABSTRACT: The ?-sheet is the key structure underlying the excellent mechanical properties of spider silk. However, the comprehensive mechanism underlying ?-sheet formation from soluble silk proteins during the transition into insoluble stable fibers has not been elucidated. Notably, the assembly of repetitive domains that dominate the length of the protein chains and structural features within the spun fibers has not been clarified. Here we determine the conformation and dynamics of the soluble precursor of the repetitive domain of spider silk using solution-state NMR, far-UV circular dichroism and vibrational circular dichroism. The soluble repetitive domain contains two major populations: ~65% random coil and ~24% polyproline type II helix (PPII helix). The PPII helix conformation in the glycine-rich region is proposed as a soluble prefibrillar region that subsequently undergoes intramolecular interactions. These findings unravel the mechanism underlying the initial step of ?-sheet formation, which is an extremely rapid process during spider silk assembly.

SUBMITTER: Oktaviani NA 

PROVIDER: S-EPMC5974136 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Conformation and dynamics of soluble repetitive domain elucidates the initial β-sheet formation of spider silk.

Oktaviani Nur Alia NA   Matsugami Akimasa A   Malay Ali D AD   Hayashi Fumiaki F   Kaplan David L DL   Numata Keiji K  

Nature communications 20180529 1


The β-sheet is the key structure underlying the excellent mechanical properties of spider silk. However, the comprehensive mechanism underlying β-sheet formation from soluble silk proteins during the transition into insoluble stable fibers has not been elucidated. Notably, the assembly of repetitive domains that dominate the length of the protein chains and structural features within the spun fibers has not been clarified. Here we determine the conformation and dynamics of the soluble precursor  ...[more]

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