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Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation.


ABSTRACT: Ductal epithelium is primarily detected in porcine neonatal pancreatic cell clusters (NPCCs) bearing grafts, suggesting that transplants might exhibit progenitor-like phenotypes. Here we found that soon after NPCC isolation, PDX1+/insulin- and SOX9+ pancreatic progenitor-like cells dramatically increased while dual-hormonal progenitor-like cells were routinely observed in NPCC culture. After transplantation (Tx), insulin+ cells increased and PDX1+ and SOX9+ cells gradually decreased in both non-diabetic (NDM) and streptozotocin-induced diabetic (DM) grafts over 2 months. Strikingly, a significantly higher percentage of insulin+ cells were detected in 9-day and 16-day, but not in 23-day, 30-day and 60-day grafts implying that hyperglycemia could only facilitate NPCC-derived ? cells early post-Tx. A higher percentage of NPCC-derived ? cells in early DM grafts was determined via an enhanced neogenic differentiation based on the detection of insulin+ cells budding out from PDX1+/SOX9+ epithelium. Interestingly, a drop in SOX9+ progenitor-like cells was detected 16 days post-Tx in DM grafts whilst PDX1+ cells do not show a significant difference until 60 days post-Tx between DM and NDM grafts, demonstrating that distinct progenitor-like populations fuel new ? cells post-Tx. In conclusion, PDX1+/SOX9+ cells could be quickly activated after NPCC isolation, maintain their multipotency in culture and differentiate into new ? cell post-Tx.

SUBMITTER: Li WC 

PROVIDER: S-EPMC5974285 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation.

Li Wan-Chun WC   Chen Chen-Yi CY   Kao Chen-Wei CW   Huang Pei-Chun PC   Hsieh Yi-Ta YT   Kuo Tz-Yu TY   Chen Tsai-Ying TY   Chia Hao-Yuan HY   Juang Jyuhn-Huarng JH  

Scientific reports 20180529 1


Ductal epithelium is primarily detected in porcine neonatal pancreatic cell clusters (NPCCs) bearing grafts, suggesting that transplants might exhibit progenitor-like phenotypes. Here we found that soon after NPCC isolation, PDX1<sup>+</sup>/insulin<sup>-</sup> and SOX9<sup>+</sup> pancreatic progenitor-like cells dramatically increased while dual-hormonal progenitor-like cells were routinely observed in NPCC culture. After transplantation (Tx), insulin<sup>+</sup> cells increased and PDX1<sup>+  ...[more]

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