Children's screen time alters the expression of saliva extracellular miR-222 and miR-146a.

ABSTRACT: An imbalance between energy uptake and energy expenditure is the most important reason for increasing trends in obesity starting from early in life. Extracellular miRNAs are expressed in all bodily fluids and their expression is influenced by a broad range of stimuli. We examined whether screen time, physical activity and BMI are associated with children's salivary extracellular miR-222 and miR-146a expression. In 80 children the extracellular fraction of saliva was obtained by means of differential centrifugation and ultracentrifugation. Expression levels of miR-222 and miR-146a were profiled by qPCR. We studied the association between children's salivary extracellular miRNA expression and screen time, physical activity and BMI using mixed models, while accounting for potential confounder

SUBMITTER: Vriens A 

PROVIDER: S-EPMC5974392 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature