Dataset Information

Difficulty with Gordonia bronchialis identification by Microflex mass spectrometer in a pacemaker-induced endocarditis.

ABSTRACT: Introduction:This report describes the first case, to the best of our knowledge, of pacemaker-induced endocarditis due to Gordonia bronchialis. Presentation:Pacemaker-induced endocarditis due to G. bronchialis infection was determined in a 92-year old man. This Gram-positive bacillus failed to be identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology, whereas the taxon was indexed in the database. 16S rRNA and rpoB gene sequencing were required to determine the correct strain identity. Conclusion:Infections caused by G. bronchialis remain a rare phenomenon affecting immunocompromised patients and/or medical device carriers. Molecular tools may be necessary to ensure accurate identification.

SUBMITTER: Titecat M

PROVIDER: S-EPMC5974928 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Difficulty with <i>Gordonia bronchialis</i> identification by Microflex mass spectrometer in a pacemaker-induced endocarditis.

Titécat Marie M Loïez Caroline C Courcol René J RJ Wallet Frédéric F

JMM case reports 20140901 3

<h4>Introduction</h4>This report describes the first case, to the best of our knowledge, of pacemaker-induced endocarditis due to <i>Gordonia bronchialis</i>.<h4>Presentation</h4>Pacemaker-induced endocarditis due to <i>G. bronchialis</i> infection was determined in a 92-year old man. This Gram-positive bacillus failed to be identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology, whereas the taxon was indexed in the database. 16S rRNA and <i>rpoB</i ...[more]

PMID: 29854410

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Project description:In patients with chronic obstructive pulmonary disease, respiratory infections are of various aetiology, predominantly viral and bacterial. However, due to structural and immunological changes within the respiratory system, such patients are also prone to mycobacterial and other relatively rare infections. We present the 70-year old male patient with chronic obstructive pulmonary disease (COPD) and coexisting bronchial asthma, diagnosed due to cough with purulent sputum expectoration lasting over three months. The first microbiological investigation of the sputum sample revealed the growth of mycobacteria. The identification test based on protein MPT64 production indicated an organism belonging to NTM (nontuberculous mycobacterium). However, further species identification by genetic testing verified the obtained culture as not belonging to the Mycobacterium genus. Based on observed morphology, the new characterisation identified an aerobic actinomycete, possibly a Nocardia spp. The isolated strain was recultured on standard microbiological media. The growth of colonies was observed on Columbia blood agar plates and solid Löewenstein-Jensen medium. The Gram and Zhiel-Nielsen stains revealed the presence of Gram-positive acid-fast bacilli. The extraction protocol and identification were performed in two repetitions; the result was G. bronchialis, with a confidence value of 99% and 95%, respectively. The gene sequencing method was applied to confirm the species affiliation of this isolate. The resulting sequence was checked against the 16S ribosomal RNA sequences database (Bacteria and Archaea). The ten best results indicated the genus Gordonia (99.04-100%) and 100% similarity of the 16S sequenced region was demonstrated for Gordonia bronchialis. The case described indicates that the correct interpretation of microbiological test results requires the use of advanced microbiology diagnosis techniques, including molecular identification of gene sequences. From a clinical point of view, Gordonia bronchialis infection or colonization may present a mild course, with no febrile episodes and no significant patient status deterioration and thus, it may remain undiagnosed more often than expected.

Dataset Information

Difficulty with Gordonia bronchialis identification by Microflex mass spectrometer in a pacemaker-induced endocarditis.

Publications

Difficulty with <i>Gordonia bronchialis</i> identification by Microflex mass spectrometer in a pacemaker-induced endocarditis.

Similar Datasets

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